Sex determination in plants and animals

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WO cytogenetic mechanisms of sex determination, the X/Y system of Mel-. Tandrium and animals and among the dioecious seed plants. WESTERGAARD. Dosage-dependent sex determination Sex determination system where the most animals, some plants and even some unicellular organ- isms. But does. Sex inheritance and sex chromosomes in plants are strikingly similar to those in animals. The majority of plants studied have heterozygous.

As described above, the sex-determining pathways in animals (or at least in their large. PDF | Sex determination systems in plants have evolved many times Correspondence: D Charlesworth, Institute of Cell, Animal and Popu-. A sex-determination system is a biological system that determines the development of sexual In animals this is often accompanied by chromosomal differences, generally through combinations of XY, ZW, XO, ZO chromosomes, or haplodiploidy. Some species such as various plants and fish do not have a fixed sex, and.

A sex-determination system is a biological system that determines the development of sexual In animals this is often accompanied by chromosomal differences, generally through combinations of XY, ZW, XO, ZO chromosomes, or haplodiploidy. Some species such as various plants and fish do not have a fixed sex, and. Sex chromosomes in plants and animals and mating type loci in fungi share strikingly similar genomic features and evolutionary processes. Summary A major reason for studying plant sex chromosomes is that they may often be 'young' systems. There is considerable evidence for the.

Chromosome Research. Our understanding of the evolution of sex chromosomes has increased greatly in recent years due to a number of molecular evolutionary investigations in divergent sex chromosome systems, and these findings are reshaping theories of sex chromosome evolution.

In particular, the dynamics of the sex-determining plants SDR have been demonstrated by recent findings in ancient and incipient sex chromosomes.

Radical changes in genomic structure and gene content in the male specific region of the Y chromosome between human and chimpanzee indicated rapid evolution in the past 6 million years, defying the notion that the pace of evolution in the SDR was fast at early stages but slowed down overtime.

The chicken Z and the human X chromosomes appeared to have acquired testis-expressed genes animals expanded in intergenic regions. Transposable sex greatly contributed to SDR expansion and aided the trafficking of animals in the SDR and its X or Z counterpart through retrotransposition.

Dosage compensation is not a destined consequence of sex chromosomes as once thought. And X-linked microRNA genes escape silencing and are expressed in testis. Collectively, these findings are challenging many of our preconceived ideas of the evolutionary trajectory and fates plants sex chromosomes. The enormous diversity of life forms on earth has been driven in part by the processes of sex and recombination that evolved in single-celled organisms and amplified in multicellular organisms.

The primary advantage of sexual reproduction is increased genetic diversity proposed by Weismannand it was validated by empirical data more than a century later Goddard plants al. The ultimate form of sexual reproduction that maximizes genetic diversity is dioecy in plants or gonochorism in animals, which is evolved by random mutation resulting in male-sterile or female-sterile phenotypes in hermaphroditic progenitors at least in plants Charlesworth and Charlesworth ; Charlesworth Some dioecious species are controlled by sex determination genes in the sex and others by sex chromosomes.

Those species with autosomal sex determination genes could acquire reverse mutations and change back to hermaphroditic forms. Others with sex chromosomes at advanced stages would remain dioecious as long as the sex chromosomes exist Ming et al. Recent findings in emerging sex chromosomes in flowering plants and fish validated their origin from autosomes Liu et al. Determination chromosomes might evolve from two closely linked sex determination genes or two linked genes, one for sex determination and the other with a sex-specific function Charlesworth and Charlesworth Suppression of recombination at the two linked genes is the pivotal event of sex chromosome evolution.

Once the recombination is suppressed, the sex-determining region SDR starts accumulating sex and other repetitive sequences, degenerating gene content, and expanding the SDR region.

Using plants as examples, the XY system evolved from a recessive mutation of a stamen-promoting gene that resulted in an intermediate gynodioecious female and hermaphrodite population, and a second gain of function mutation that occurred on the same chromosome, in close proximity to the functional stamen-promoting gene, which resulted in carpel suppression Charlesworth and Charlesworth Animals XY system is male heterogametic and Y is the dominant sex-determining chromosome.

In the ZW system, females are heterogametic and the W is the dominant sex-determining chromosome. In plants, the female specifying W contains a dominant male sterility locus permanently linked to a dominant female-promoting locus. Whether female heterogamety in plants evolves through an androdioecious or a gynodioecious intermediate depends on the nature of the first mutation.

The UV system evolved in algae and bryophytes with a predominant haploid phase in their life cycle. Females, which make large gametes, are determined by a U chromosome and males, which make small gametes, are determined by a V chromosome. The diploid stage with UV chromosomes is therefore always heterogametic Bachtrog et al. Sex chromosomes in plants plants animals and mating type determination in fungi share strikingly similar genomic features and evolutionary processes, and this convergent evolution across kingdoms reflects similar selection forces and drive their formation Fraser et al.

Sex chromosomes at advanced stages are easily distinguishable from autosomes because they are heteromorphic from each other and from the autosomes. The size variation between the pair is caused by different rates of expansion or contraction of the sex-limited, hemizygous chromosome Y or W compared to their counterpart X or Z.

The dynamics of sex chromosome evolution are limited to the non-recombining SDR and its X or Z counterpart, whereas the pseudo-autosomal regions recombine normally like autosomes, despite elevated recombination rates near the borders of SDR Yu et al.

In this review, we sex on recent advances in the genomics of SDR and its Animals or Z counterpart, because and their defining role in sex determination and impact on human health and crop improvement.

Comparative organization of SDR in selected species. The variation of size is a plants process at different stages of sex chromosome evolution.

The two vertical lines in the proto W chromosome determination the two sex determination genes. In medaka fish, the sex determination gene dmrt1bY was duplicated from the ancestral dmrt1a gene on LG9 and translocated to the Y chromosome.

The small size of the medaka MSY is likely restricted by the unique structure of the flanking borders, where duplicated determination OlaflnkL and OlaflnkR are located. Either sex these two genes can recombine with the single sex X counterpart OlaflnkX. The medaka MSY consists of a The Chromosomal rearrangements are also animals in the SDR.

And are often the molecular basis of recombination suppression. Five evolutionary strata in the human X chromosome were created by five inversion events on the Y chromosome, although there is no direct evidence except the pericentric inversion shown in elephant X chromosome Lahn and Page ; Sex et al.

Three evolutionary strata triggered by three progressive inversions are also found in the chicken Z chromosome Nam and Ellegren The eight palindromes animals the human MSY plants inverted duplications, and such structure has not been found in the recently evolved poplar W and papaya And chromosomes Yin et al.

Ming unpublished data. There are 19 palindromes in the chimpanzee Animals Hughes et al. Sex them, seven are shared with the human MSY, perhaps and from the ancestral Y chromosome, and 12 are chimpanzee-specific.

Moreover, most palindromes in the chimpanzee MSY exist in multiple copies, resulting in a higher rate of arm-to-arm sequence divergence in some chimpanzee palindromes compared to that of single-copy palindromes in the human MSY. Small-scale local rearrangements were hard to find in the human X and Y since the Y chromosome had gone through the degeneration processes with hardly any traces of local rearrangement remaining.

Such local rearrangements were numerous between the X and Y chromosomes in papaya, including inversions, insertions, deletions, duplications, and translocation Yu et al. Ming unpublished. Inversions and deletions were also found in the Y chromosome of three-spined stickleback fish Ross and Peichel Numerous chromosomal rearrangements were found between human and animals Y chromosomes, whereas the human and chimpanzee chromosome 21 were collinear Hughes et al.

The rapid divergence of the SDR offers an opportunity to test the existence of sex chromosomes in dioecious species using next-generation sequencing technologies. For instance, it has been controversial whether sex chromosomes have and in dioecious date palm. Three male and six female date palm genomes were sequenced using Illumina with sequence coverage ranging from Single-nucleotide polymorphism SNP analysis revealed that 1, out of 3.

Heterozygous SNP genotypes were found in all male genomes and homozygous genotypes in all female genomes, indicating that date palm indeed has XY chromosomes.

Genetic mapping of SNPs in the four scaffolds containing the largest number of SNPs showed that all four scaffolds are linked with no recombination. This approach could be used to quickly analyze the SDRs of dioecious species with limited genomic resources. However, nearly all de novo genome sequencing projects were carried out using the homogametic sex to avoid the difficulty of assembling the heterozygous SDR region, although heterogametic sex genotypes were used for re-sequencing projects.

The only exception is the poplar genome sequencing project that used a female plant, but later discovered that poplar has a ZW sex chromosome system Tuskan et al. Currently, sequencing of the SDR and its Plants or Z determination is complete for human, medaka fish, poplar, and papaya Skaletsky et al. The most extensively sex sex chromosomes are those in human. The human Y chromosome lost most of the 1, genes present in the X chromosome Ross et al.

The MSY contains 78 protein coding genes that encode 27 different proteins Skaltsky et al. Out of the 78 genes, only 16 The paired genes on the Y determination diverged, and each encodes a different protein.

These 16 X-degenerated genes encode 16 proteins. The remaining 60 genes are in the eight palindrome sequences, and some have orthologs in determination X chromosomes, such as RBMX Delbridge et al. These 60 ampliconic genes are in 9 gene families and encode 9 distinctive proteins that are expressed in the testis.

Moreover, the human MSY includes 78 additional transcription units without strong evidence of being protein coding, including 13 single-copy units and 65 units in 15 MSY-specific families. Taken together, human MSY gained The complete sequencing of sex chimpanzee Y chromosome provided a second well-characterized and Y chromosome to examine the loss and gain of genes within a 6 million year time frame Hughes et al.

The chimpanzee MSY contains 37 genes coding for 18 different proteins, including 12 X-degenerated sex 25 ampliconic genes. It is not clear whether gorilla retains additional X-degenerated genes plants are lost in the human MSY since the gorilla Y chromosome has not been fully sequenced. Chimpanzee also lost 3 of the 9 gene families by frameshift mutations, albeit it has 11 more palindromes than those in human, with 25 genes animals 6 gene families remaining.

No new ampliconic genes are found in chimpanzee, suggesting that the nine ampliconic gene families were likely acquired from autosomes before the divergence of human and chimpanzee.

One gene family, TSPY plants, has 35 members in human, but only 6 in chimpanzee, perhaps due to expansion in human. The gene loss in the human MSY is so extensive that it is animals to assess how many of the 1, genes in the X chromosomes are from the ancestral autosomes. Young sex chromosomes in flowering plants and fish fill in the gap to document the gene gains and losses of the Determination in the early stage of sex chromosome evolution.

The medaka Y chromosome, for example, has an MSY restricted in a small kb region because of the pair of duplicated genes in the borders, formed by an insertion determination a duplicated fragment of LG9 into the current Y chromosome, thus having no homologous sequence in the X chromosome.

The ancestral The MSY contains only one functional gene, the sex determination gene dmrt1bYand four pseudogenes, showing the loss of three genes that are still functioning in LG9 Kondo et al.

The poplar W chromosome is also unique. Papaya plants chromosomes are more conventional, with an 8. The liverwort V previously referred to as Y chromosome was sequenced, and 64 genes were annotated in the Mb V chromosome Yamato et al. Nevertheless, 14 of the 64 genes are V-specific, indicating that gene gains have occurred in plants liverwort V chromosome. The X chromosome has been thought to remain relatively stagnant and to conserve the structure of the autosome from which the sex chromosomes originally arose Ohno ; Bull Determination, additional findings on the X chromosome have brought to light a more complex scenario.

Over time, the X chromosome has been altered and shaped by selection pressures, creating a distinctly different chromosome than its ancestral counterpart. One such way, the X chromosome has evolved is by gene trafficking.

The therian and Drosophila X chromosomes both selectively lost genes, many of which function during meiosis, as well as gained sex-biased genes Potrzebowski et al. And young X chromosome went through increased bouts of adaptive evolution and demasculinization of its gene content Bachtrog et al.

In mammals, retrotransposed copies of important housekeeping genes were fixed into the autosome, possibly as a way to compensate for the Determination parental gene being silenced during and after meiosis animals meiotic sex chromosome inactivation MSCI Potrzebowski et al. MSCI is the heterochromatinization of the X and Y chromosomes during meiosis, which silences transcription. Silencing crucial housekeeping genes on the X could be detrimental, but autosomal retrotransposed copies of the vital silenced X genes compensate for that loss.

This type of adaptive evolution is also seen in Drosophilawhere meiotic testis-expressed genes were retrotransposed from the X chromosome to the autosome, suggesting MSCI may influence gene content on and X chromosome Vibranovski et al.

Evolution of reproductive systems in the genus Silene. Proc R Soc B , : — Isolation of Y chromosome-specific sequences from Silene latifolia and mapping of male sex determining genes using representational difference analysis.

Sexual dimorphism in white campion: complex deletion on the Y chromosome results in a floral asexual type. DNA diversity in sex linked and autosomal genes of the plant species Silene latifolia and S. Molec Biol Evol , 18 : — Evolution of a plant Y-chromosome: variability in a Y-linked gene of Silene latifolia.

Nature , : — Different kinds of male flowers in the dioecious plant Asparagus officinalis L. Sex Plant Reprod , 6 : 16— Restricted gene flow and subpopulation differentiation in Silene dioica.

Heredity , 80 : — Genetics of sex determination in flowering plants. Devel Genet , 15 : — Guttman, DS, Charlesworth, D An X-linked gene has a degenerate Y-linked homologue in the dioecious plant Silene latifolia. Haigh, J The accumulation of deleterious genes in a population. Theor Pop Biol , 14 : — Comparison of MADS box gene expression in developing male and female flowers of the dioecious plants white campion.

Plant Cell , 6 : — Sex determination in Actinidia. Sex-linked markers and progeny sex ratio in diploid A. Sex Plant Repro , 10 : — Heilbuth, JC Lower species richness in dioecious clades. Kin-structured colonization and small-scale genetic differentiation in Silene dioica.

Evolution , 53 : — Four evolutionary strata on the human X chromosome. Science , : — Sexual dimorphism in white campion: complex control of carpel number is revealed by Y chromosome deletions. Lengerova, M, Vyskot, B Sex chromatin and nucleolar analyses in Rumex acetosa L. Protoplasma , : — Lewis, D The evolution of sex in flowering plants. Biolog Rev , 17 : 46— Lloyd, DG Female-predominant sex ratios in angiosperms. Heredity , 32 : 35— Lloyd, DG a.

Breeding systems in Cotula. Dioecious populations. New Phytol , 74 : — Lloyd, DG b. The transmission of genes via pollen and ovules in gynodioecy angiosperms. Theoret Pop Biol , 9 : — Gender allocations in outcrossing cosexual plants. Sinauer: Sunderland, Mass, pp — Modification of the gender of seed plants in varying conditions. Evol Biol , 17 : — Theoret Appl Genet , 98 : 86— Isolation and developmental expression of male reproductive organ-specific genes in a dioecious campion, Melandrium album Silene latifolia.

Plant J , 10 : — McCauley, DE Contrasting the distribution of chloroplast DNA and allozyme polymorphism among local populations of Silene alba : implications for studies of gene flow in plants.

Muller, HJ The relation of recombination to mutational advance. Mut Res , 1 : 2—9. Nei, M Molecular Evolutionary Genetics. Columbia University Press: New York. Nordborg, M A model of genetic modification in gynodioecious plants. Proc Roy Soc Lond B , : — The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene. Parker, JS Sex-chromosome and sex differentiation in flowering plants.

Chromosomes Today , 10 : — Identification of sex in hop Humulus lupulus using molecular markers. Cytogenetic and molecular analysis of the multiple sex-chromosome system of Rumex acetosa.

Heredity , 72 : — Dioecy and its correlates in the flowering plants. Am J Bot , 82 : — Rice, WR Genetic hitch-hiking and the evolution of reduced genetic activity of the Y sex chromosome. The accumulation of sexually antagonistic genes as a selective agent promoting the evolution of reduced recombination between primitive sex-chromosomes. Evolution , 41 : — The influence of population size and isolation on gene flow by pollen in Silene alba.

Evolution , 53 : 63— The effect of hitchhiking on genes linked to a balanced polymorphism in a subdivided population. Genet Res , 76 : 63— High-stringency subtraction for the identification of differentially regulated cDNA clones. Biotechniques , 23 : Smith, BW The mechanism of sex determination in Rumex hastatulus.

Genetics , 48 : — Mol Biol Evol , 16 : — Taylor, DR Sex segregation ratio and gender expression in the genus Actinidia. Sex Plant Repr , 8 : — Traut, W, Willhoeft, U A jumping sex determining factor in the fly Megaselia scalaris.

Chromosoma Berl , 99 : — The development of male and female regenerants by in-vitro androgenesis in dioecious plant Melandrium album. Ann Bot , 73 : — DNA methylation of sex chromosomes in a dioecious plant, Melandrium album.

Mol Gen Genet , : — Euchromatic domains in plant chromosomes as revealed by H4 histone acetylation and early DNA replication. Genome , 42 : — Wang, J Effective size and F-statistics of subdivided populations for sex-linked loc. Theoret Pop Biol , 55 : — The human Y chromosome derives largely from a single autosomal region added to the sex chromosomes 80— million years ago.

Cytogenet Cell Genet , 92 : 74— A phylogenetic analysis of Schiedia and Alsinidendron Caryophyllaceae: Alsinoideae : implications for the evolution of breeding systems. Syst Bot , 20 : — Evidence for genetic drift in endosymbionts Buchnera : analyses of protein-coding genes.

Mol Biol Evol , 16 : 83— Westergaard, M The mechanism of sex determination in dioecious plants. Adv Genet , 9 : — Continuous variation in Y-chromosome structure of Rumex acetosa. Heredity , 57 : — Mendelian and non-Mendelian inheritance of newly-arisen chromosome rearrangements. Heredity , 60 : — Sex determination in the dioecious Melandrium. First lessons from androgenic haploids. Sex Plant Repr , 3 : — Y chromosome specific markers and the evolution of dioecy in the genus Silene.

Download references. Correspondence to D Charlesworth. Reprints and Permissions. Charlesworth, D. Plant sex determination and sex chromosomes. Heredity 88, 94— doi Download citation. Journal of Experimental Botany The American Naturalist Genome Research BMC Plant Biology Scientific Reports Advanced search.

Skip to main content. Abstract Sex determination systems in plants have evolved many times from hermaphroditic ancestors including monoecious plants with separate male and female flowers on the same individual , and sex chromosome systems have arisen several times in flowering plant evolution.

Access through your institution. Buy or subscribe. Download PDF. Introduction: why are plant sex chromosomes of particular interest? Table 1 Sex and gender systems of sexually reproducing flowering plants Full size table. Figure 1. Full size image. The genetics of sex determination in plants, and plant sex chromosomes Sex inheritance and sex chromosomes in plants are strikingly similar to those in animals.

Figure 2. Why are sex determining loci linked? Figure 3. Evolution of sex chromosomes The theory outlined here explains the evolution of a rarely recombining chromosome region containing the sex determining genes, an incipient sex chromosome system.

Have plant Y chromosomes degenerated? Molecular genetics of plant Y chromosomes Our understanding of the evolution of plant sex chromosomes and sex determination should be advanced by the use of molecular markers, so several groups are searching for these. Discussion With the availability of molecular techniques, we may now hope to understand more about how sex chromosomes evolve.

Google Scholar Caballero, A Google Scholar Charlesworth, D Google Scholar Charlesworth, B Google Scholar Darwin, CR Google Scholar Heilbuth, JC Google Scholar Lloyd, DG Google Scholar Lloyd, DG a. Google Scholar Lloyd, DG b. Google Scholar Nei, M Google Scholar Nordborg, M Google Scholar Rice, WR Google Scholar Westergaard, M Rights and permissions Reprints and Permissions.

About this article Cite this article Charlesworth, D. Coelho , Michael E. Siena , Juan Pablo A. Heredity menu. Nature Research menu. We therefore decided to look for the orthologs in Silene latifolia , of two central genes in meristem function in A. We performed comparative in situ hybridization on young flower buds from male, female and hermaphrodite mutant plants obtained by deleting portion of the Y chromosome 9 , using SlSTM and SlCUC as probes.

In addition, our results reveal a clear difference in the pattern of expression between males and females or hermaphrodites, before any morphological difference become apparent. These observations reflect an early arrest of meristem function in male flowers and could be the cause of the lack of cell divisions observed. Our results make SlSTM and SlCUC strong candidates for being involved in sex determination in Silene latifolia and therefore open new perspective for molecular mechanism of sex determination in plants in general.

National Center for Biotechnology Information , U. Journal List Plant Signal Behav v. Plant Signal Behav. Author information Article notes Copyright and License information Disclaimer. Corresponding author.

Received Dec 19; Accepted Dec Abstract Most dioecious plant species are believed to derive from hermaphrodite ancestors. Key Words: floral meristem, sex determination, shoot-meristemless, cup shaped cotyledon.

Sex Determination in Plants Mechanisms underlying sex determination in plants are largely unknown. Candidate Genes Since we observed a whorl-specific arrest in cell proliferation in the early male flower meristem, we suspected a precocious arrest of the flower meristem in male individuals.

References 1. Guttman DS, Charlesworth D. An X-linked gene with a degenerate Y-linked homologue in a dioecious plant. SlY1 , the first active gene cloned from a plant Y chromosome, encodes a WD-repeat protein. EMBO J. Low variability in a Y -linked plant gene and its implications for Y-chromosome evolution. A gradual process of recombination restriction in the evolutionary history of the sex chromosomes in dioecious plants. PLoS Biol. Comparison of MADS box gene expression in developing male and female flowers of the dioecious plant white campion.

Plant Cell.