Parasexual hybridization

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Parasexual hybridization can be used where sexual hybridation is un successful.​Transduction and transformation are well known methods of. is a nonsexual mechanism of transferring genetic material by fusion of cells of different characteristics. Protoplast fusion technique is used for. Curr Genet. Nov;4(2) doi: /BF Parasexual hybridization of yeasts by electric field stimulated fusion of protoplasts. Weber H(​1).

Parasexual Interspecific Plant Hybridization. (Nicotiana/leaf mesophyll/plant tissue culture/genetics/selective media). PETER S. CARLSON, HAROLD H. SMITH. is a nonsexual mechanism of transferring genetic material by fusion of cells of different characteristics. Protoplast fusion technique is used for. Parasexual hybridization can be used where sexual hybridation is un successful.​Transduction and transformation are well known methods of.

Parasexual hybridization is a nonsexual mechanism of transferring genetic material by fusion of cells of different characteristics. Protoplast. Abstract. Interspecific plant hybrids have been produced by parasexual procedures. Protoplasts of Nicotiana glauca and N. langsdorffii were isolated, fused, and. Parasexual Interspecific Plant Hybridization. (Nicotiana/leaf mesophyll/plant tissue culture/genetics/selective media). PETER S. CARLSON, HAROLD H. SMITH.






Protoplasts are single cells that have hybridization stripped of parasexual wall exposing the plasmalemma. A special property of the protoplasts hybridization that when brought into hybridization contact they tend to fuse with each other irrespective of the sources of the protoplasts.

The technique of fusion of isolated protoplasts from parasexual cells and regeneration of hybrid plants from the fusion products, called somatic hybridization or parasexual hybridization completely bypasses the sex and, thus, allows combining hybridization of two desirable parents, irrespective of their taxonomic relationship.

The technique of cell fusion has been applied to produce a large number of intergeneric, intertribal or interfamily symmetric allotetraploid parasexual hybrids, asymmetric hybrids and somatic hybrids. According to Liu et al 90 intergeneric somatic hybrids have been obtained. This chapter describes the techniques of enzymatic isolation of protoplasts in parasexual quantities, their fusion chemically and electrically, regeneration of somatic hybridization from the fused somatic cells, and application of parasexual hybridization.

Skip parasexual main content. Advertisement Hide. Chapter First Online: 21 March hybridization This process is experimental and the keywords may be updated as the learning algorithm improves. This is a preview of subscription content, log in to check access. Cocking EC A method for the isolation of plant protoplasts and vacuoles. Hybridization — Google Scholar. Plant Breed Rev — Google Scholar. Wiley, U.

K Parasexual Google Scholar. K Google Scholar. Planta — CrossRef Google Scholar. Euphytica — CrossRef Google Scholar. Naturwissenschaften — Parasexual Google Scholar. Zimmermann U, Scheurich P High frequency fusion of plant protoplasts by electric fields. Parasexual —32 CrossRef Google Parasexual. Personalised recommendations.

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Euphytica — CrossRef Google Scholar. Naturwissenschaften — CrossRef Google Scholar. Zimmermann U, Scheurich P High frequency fusion of plant protoplasts by electric fields.

Planta —32 CrossRef Google Scholar. Personalised recommendations. Cite chapter How to cite? ENW EndNote. Buy options. Copyright notice. This article has been cited by other articles in PMC. Abstract Interspecific plant hybrids have been produced by parasexual procedures. Images in this article Image on p. Image on p. Fusion of isolated plant protoplasts. Plant Physiol. Nevertheless, the result will be the same when it does occur—the recombination of genes.

Such nuclei with incomplete multiples of the haploid number are termed aneuploid , as they do not have even chromosome number sets such as n or 2n. Consistent with this, in E. Each of these events is relatively rare, and they do not constitute a regular cycle like the sexual cycle. But the outcome would be similar. Once a diploid nucleus has formed by fusion of two haploid nuclei from different parents, the parental genes can potentially recombine.

And, the chromosomes that are lost from an aneuploid nucleus during its reversion to a euploid could be a mixture of those in the parental strain. The potential to undergo a parasexual cycle under laboratory conditions has been demonstrated in many species of filamentous fungi, including Fusarium monoliforme , [3] Penicillium roqueforti [4] used in making blue cheeses [5] , Verticillium dahliae , [6] [7] Verticillium alboatrum , [8] Pseudocercosporella herpotrichoides , [9] Ustilago scabiosae , [10] Magnaporthe grisea , [11] Cladosporium fulvum , [12] [13] and the human pathogens Candida albicans [14] and Candida tropicalis.

Parasexuality has become a useful tool for industrial mycologists to produce strains with desired combinations of properties. Its significance in nature is largely unknown and will depend on the frequency of heterokaryosis, determined by cytoplasmic incompatibility barriers and it is also use full in rDNA technology. From Wikipedia, the free encyclopedia.

Fungal Biology. Cambridge, MA: Blackwell Publishers. Current Genetics.